The low G-C posts informs us that piece of DNA has actually an excellent lowest melting section and that’s a lot more open to polymerases. Chargaff’s regulations help us dictate that the DNA should be 15% guanine and fifteen% cytosine.
We including be aware that other nucleotide blogs when you look at the the new DNA must be including adenine and you will thymine (since the DNA features 2 kinds of nucleotide bonds). Hence, the brand new part of combined adenine and you will thymine posts must be 70%, or thirty-five% each.
We have now discover our DNA fragment consists of fifteen% guanine, 15% cytosine, 35% adenine, and you can thirty five% thymine. When we range from the percent of purine nucleotides along with her and pyrimidine nucleotides together with her such as Chargaff’s rule, we get 50% purine articles and you can 50% pyrimidine articles: ultimately causing a 1-to-step one proportion, just as Chargaff discussed.