7 metabolites was indeed split up by thin layer chromatography and you may developed which have benzene:acetone (4:step one). step one, E1; 2, AD; step three, P5; cuatro, DHEA 17-P; 5, E2; six, 17-P5; eight, 17,20?-P 17,21-P; and you can 8, 17,20?-P5. Discover Desk 1 for steroid abbreviations.
Desk step three
To find the steroidogenic pathway off E2 synthesis, four most other radiolabeled steroid drugs were used as the precursors to have within the vitro incubation regarding follicles. Of these four precursors, E2 was synthesized off [ fourteen C]DHEA, [ fourteen C]Ad, and you may [ 3 H]E1. Whenever hair follicles was indeed incubated which have [ step three H]17-P, E2 was not seen as the metabolite, and you may a small amount of Advertisement try put. In most incubations, T production could not be observed, and you will [ 14 C]T was not totally transformed into E2.
Aspect regarding serum steroid accounts
Gel degrees of E2 and E1 shown comparable pages into the an excellent day of spawning season (Fig. dos). During the hr, if developmental phase of the biggest oocytes try LMN, serum E2 and you will E1 accounts have been somewhat large (E2= ng/ml, E1= 5.08 ng/ml). But from the hours, if the phase of your biggest oocytes are Meters, the amount decrease (E2=5.03 ng/ml, Estep one=1.50 ng/ml) and you can stayed lingering levels on and time, if the degrees of the biggest oocytes had been TY and you can EMN, correspondingly (E2=6.00 ?six.twenty-seven ng/ml, E1=step one.step 15–step 1.79 ng/ml). However, T and you can Post levels was lingering (T=step one.22–step 1.71 ng/ml, AD=1.23–step 1.43 ng/ml) all the time and you may don’t disagree significantly one of ovarian grade (Fig. step three).
Fig. 2
Changes in the serum levels of estradiol-17? (E2) and estrone (E1) in female bambooleaf wrasse sampled at different times of a day during the spawning season.